Biochemical and Histological Evaluation of Sub-Chronic Administration of Canscora decussate Leaf Extract on Liver and Heart of Wistar Rats

Authors

  • Opeyemi T.  Joseph Departmennt of Pharmacology, Faculty of Pharmacy, Lead city University, Ibadan Oyo State, Nigeria.
  • Oyepata S. Joseph Departmennt of Pharmacology, Faculty of Pharmacy, Lead city University, Ibadan Oyo State, Nigeria.
  • Adekunle T.  Adegbuyi Departmennt of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Federal University, Oye–Ekiti, Ekiti State, Nigeria.

DOI:

https://doi.org/10.26538/tjpps/v3i7.4

Keywords:

Canscora decussate, Blood, Rats, Liver, Heart

Abstract

Plants have been used as food and medicine since the beginning of recorded human history. Canscora decussate is traditionally believed to relieve cardiovascular and hepatic disorder. The aim of this study is to evaluate the biochemical and histological effect of sub-chronic administration of ethanol leaf extract of Canscora decussate on liver and heart of wistar rats. Twenty four male adult rats were used for this work. Group 1 received distilled water (10 ml/kg), while groups 2, 3, and 4 received 100, 200, and 400 mg/kg of Canscora decussate, respectively. The animals were housed in conventional cages for 28 days, with oral access to the extract provided before they were weighed and euthanized. A heart puncture was used to take blood, which was then promptly submitted for chemopathological investigation. Phytochemical analysis revealed the presence of secondary metabolite such as tannin, reduced sugar, flavanoids and saponins. There was significant decrease  (P≤0.05) in serum ALB at 200 and 400 mg/kg dose, while there was no significant change (P ≤0.05) in ALT and ALP when compared to the control group. There was significant increase (P ≤0.05) in level of CHOL at 200 mg/kg dose when compared to the control. There was significant (P ≤0.05) increase in serum HDL at 400 mg/kg dose. LDL and TRI value remain unchangeSlight lymphocyte hyperplasia with normal heart and liver histology architecture observed at all doses and the control (10 ml/kg). Canscora decussate is relatively safe for use and may be of benefit in cardiovascular and hepatic conditions.

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References

Cruchaud A, Juditz E. "An analysis of gastric parietal cell antibodies and thyroid cell antibodies in patients with pernicious anaemia and thyroid disorders". Clin Exp Immunol. 1968; 3 (8): 771–81. PMC 1578967. PMID 4180858.

De Araujo IE, Oliveira-Maia AJ, Sotnikova TD, Gainetdinov RR, Caron MG,Nicolelis MAL, Simon SA.. "Food Reward in the Absence of Taste Receptor Signaling". Neuron. 2008; 57 (6): 930–41. doi:10.1016/j.neuron.2008.01.032. PMID 18367093. S2CID 47453450.

Oyepata JS, Opeyemi JT, Adekunle AT, Moses OA. Effect of Pterocarpus santalinus Ethanol Leaf Extract on Haematological, Histopathological and Lipid Profile Indices in Wistar Rats. Trop J Nat Prod Res. 2024; 8(3):6681-6685. https://doi.org/10.26538/tjnpr/v8i3.30

Ackroff K, Lucas F, Sclafani A.. "Flavor preference conditioning as a function of fat source". Physiol. Behav. 2005; 85 (4): 448–460. doi:10.1016/j.physbeh.2005.05.006. PMID 15990126. S2CID 7875868.

Cotran RS, Kumar V, Fausto N, Robbins SL, Abbas AK. Robbins and Cotran pathologic basis of disease. St. Louis, MO: Elsevier Saunders. 2005; ISBN 978-0-7216-0187-8.

Mescher AL. Junqueira's Basic Histology, 2026; 14th edition. Lange. p. 393.

otran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; NelsoFausto; Robbins, Stanley L.; Abbas, Abul K.. Robbins and Cotran pathologic basis of disease

(7th ed.). St. Louis, MO: Elsevier Saunders. 2015; p. 878. ISBN 978-0-7216-0187-8.

Kalantar-Zadeh K, McCullough PA, Agarwal SK, Beddhu S, Boaz M, Bruchfeld A, et al. "Nomenclature in nephrology: preserving 'renal' and 'nephro' in the glossary of kidney health and disease". Jour of Neph. 2021; 34 (3): 639–648. doi:10.1007/s40620-021-01011-3. PMC 8192439. PMID 33713333.

Joseph OS, Joseph OT, Musa TL and Oyepata PJ. Histological evaluation of the nephroprotective activity of the ethanol stem extracts of Homalium letestui in Gentamicin – induced albino rats injury, using various staining techniques. GSU. 2019; Volume 7, Issue 8. Page 1065-1087.

Doniach D, Roitt IM, Taylor KB.. "Autoimmunity in pernicious anemia and thyroiditis: a family study". Ann N Y Acad Sci. 2018; 124 (2): 605–25. Bibcode:1965NYASA.124..605D. doi:10.1111/j.1749-6632.1965.tb18990.x. PMID 5320499. S2CID 39456072.

Kalantar-Zadeh K, Jafar TH, Nitsch D, Neuen BL, Perkovic V. "Chronic kidney disease" (PDF). Lancet. 2021; 398 (10302): 786–802. doi:10.1016/S0140-6736(21)00519-5. PMID 34175022. S2CID 235631509. Archived (PDF) from the original on 2022-05-17. Retrieved 2022-05-22.

Lv JC, Zhang LX. "Prevalence and Disease Burden of Chronic Kidney Disease". Renal Fibrosis: Mechanisms and Therapies. Adv in Exp Med. and Bio. 2019; Vol. 1165. pp. 3–15. doi:10.1007/978-981-13-8871-2_1. ISBN 978-981-13-8871-2. PMID 31399958. S2CID 199519437.

Kristan WB Jr, Calabrese RL, Friesen WO. "Neuronal control of leech behavior". Prog Neurobiol. 2015; 76 (5): 279–327. doi:10.1016/j.pneurobio.2005.09.004. PMID 16260077. S2CID 15773361.

Lahner E, Conti L, Cicone F, Capriello S, Cazzato M, Centanni M, Annibale B, Virili C. "Thyro-entero-gastric autoimmunity: Pathophysiology and implications for patient management. A review". Best Pract Res Clin Endocrinol Metab. 2019 33 (6): 101373.

Ethiya NK, Patel MB, & Mishra SH. Phytopharmacologic aspects of CanscoradecussataRoem and Schult. Pharmacognosy reviews, 2014; 4(7), 49–57. https://doi.org/10.4103/0973-7847.65326

Joseph OS, Joseph OT, El-Gazzar AM, Mahmoud MH, Omoirri MA, & Gaber ESB. Toxicological evaluation of ethanol leaf extract of Pterocarpus santalinus on lungs, stomach, brain and hematological parameters of Wistar rats. Cogent Food Agric , 2024; 10(1), 2303828.Link: https://www.tandfonline.com/doi/full/10.1080/23311932.2024.2303828

Chaudhuri RK, Ghosal A. Xanthones of Canscoradecussata. Phytochemistry. 1971;10:2425–32.

Ghosal S, Chaudhuri RK, Nath A. Lanostanetriterpenes of Canscoradecussata. Phytochemistry. 1973;12:1763–6.

Simeon JO, Tosin JO. Toxicological Evaluation of Sub-Chronic Administration of Ethanol Leaf Extract of Ocimum canum on Lipid Parameters, Heart Histology and Feed Consumption of Wistar Rats. Calip Jour of Sci. and Tech. Jou.; 2024;Vol. 6 No. 1. Pp. 70-76.

Oyepata JS and Adekunle AT. Haematological, Renal and Gastric Effects of Ethanol Leaf Extract of Bauhinia variegate Linn.Trop J Phytochem Pharm. Sci. 2024; 3(2):174-178. http://www.doi.org/10.26538/tjpps/v3i2.3

Hodges R, Porte AL. The structure of loliolide: A terpene from Loliumperenne. Tetrahedron. 1964;20:1463–7.

Ghosal S, Biswas K, Chaudhuri RK. Chemical constituents of Gentianaceae part 22, structure of new 1, 3, 5-tri and 1, 3, 5, 6, 7-penta oxygenated xanthonesCanscoradecussataSchult. J Pharm Sci. 1977;14:1597–605. [PubMed] [Google Scholar]

Joseph OS, Joseph OS, Sabastine AZ, AA. Concomitant administration of ethanol leaf extract of Thymus vulgaris on Diazepam– induced Sedation and Hypnosis in Wister Rat. Jour. of Nurs and Health Sci. 2021; Volume 16, Issue 5. PP 04-09.

Sethiya NK, Nahata A, Dixit VK. Comparative thin layer chromatographic investigations on commercial sources of Shankhpushpi in India. Pharmacog. Jour. 2019; 1:224–6.

Bhattacharya SK, Ghosal S, Chaudhuri RK, Sanyal AK..C.decussata (Gentianaceae) Xanthones 3 Pharmacological Studies. J Pharm. Sci. 1972;61:1838–49.

Finegan RA, Stephani RA, Ganguli G, Bhattacharya SK. Occurrence of mangiferin in HiptagemadablotaGaertn. J Pharm Sci. 2008; 57:1039.

Joseph OS, Joseph OT, Sabastine AZ. Evaluation of the Effect of Anacardium Occidentale Cashew Apple Juice on Kidney of Gentamicin Induced Injury in Albino Rats. Jour Cli and Lab Res. 2023; Volume 6(1. Page 1-8. DOI:10.31579/2768-0487/100

Paradisiaca Stem Juice on the Pancreatic Cells of Wistar Rats. Trop J Nat Prod Res. 2024; 8(1):6035-6038. http://www.doi.org/10.26538/tjnpr/v8i1.46

Okokon JE, Joseph OS, and Umoh EE. Nephroprotective activity of Homaliumletestui stem extract against paracetamol induced kidney injury. Jour. of Exp and Inte Med. 2016; Vol. 6 (1): 38-43.DOI: 10.5455/jeim.250216.or.147

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Published

2024-11-06

How to Cite

Joseph, O. T., Joseph, O. S., & Adegbuyi, A. T. (2024). Biochemical and Histological Evaluation of Sub-Chronic Administration of Canscora decussate Leaf Extract on Liver and Heart of Wistar Rats. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 3(7), 380 – 385. https://doi.org/10.26538/tjpps/v3i7.4