<i>In-vitro</i> Anti-inflammatory and Antioxidant Potentials of Methanol Extract of&nbsp;<i>Uvaria chamae</i>&nbsp;(Bush Banana) Leaves



  • Godfrey R. Kweki Department of Medical Biochemistry, Delta State University, P.M.B. 1, Abraka
  • Anthony Orhu School of Science and Technology, Delta State School of Marine Technology, Burutu,
  • Ugochukwu Uzuegbu Department of Medical Biochemistry, Delta State University, P.M.B. 1, Abraka
  • Okeroghene S. Iwhiwhu School of Science and Technology, Delta State School of Marine Technology, Burutu,
  • Oghenenyore A. Ohwokevwo Department of Biochemistry, Delta State University of Science and Technology, P.M.B. 5, Ozoro


U. chamae, free radicals, Anti-inflammatory, Antioxidant, Phytochemical


Plants existence is pivotal for human survival, they do not only serve as a source of foliage and economic value but also provide an alternative source of medicine in combating various metabolic disorders. The preliminary investigation of the bioactive composition, in-vitro antioxidant, and anti-inflammatory properties of methanol extract of Uvaria chamae leaves was evaluated in this study. The antioxidant activities were assessed using four various assays; Nitric oxide (NO) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities, Reducing power (RP), and Total antioxidant capacity (TOAC). In-vitro anti-inflammatory potentials were quantified using membrane stabilization, albumin denaturing test, and protease inhibitor test in addition to qualitative and quantitative plant phytochemical assays. The result obtained detected the presence of phenol, flavonoids, alkaloids, terpenes, and tannins from the qualitative phytochemistry. However, Flavonoid recorded the highest concentration of 1.21mg/gCAE while the least was alkaloid 0.41mg/gATE. The in-vitro antioxidant and anti-inflammatory activities of the extract showed a significant (p<0.05) increase in the activities of the extract in a concentration-dependent manner, while the respective IC50 values showed that the extracts obtained higher values in comparison (p<0.05) to the respective standard for the antioxidant activities. Conversely, the extract IC50 values for the anti-inflammatory activities were lower when compared (p<0.05) to the aspirin. The results of these investigations, suggest that Uvaria. chamae leave methanol extracts have antioxidant and anti-inflammatory properties. The mechanism of action may be due to its high content of flavonoids, which are known to inhibit membrane lysis, denaturation of albumin, and the activity of proteases. This study supports the utilization of Uvaria .chamae in folk medicine.


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Yesmin S, Paul A, Naz T, Rahman ABM. Akhter SF, Wahed MI, Emran TB, Siddiqui SA, Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanol root extract of Choi (Piper chaba), Clin Phytosci 2020; 6, 59

Kadiri HE, Okoro IO, Ichipi-Ifukor PC, Tetrapleura tetraptera fruit protects against cyanide induced toxicity in rats, Iraqi J. Sci. 2020; 61(10): 2504–2514.

Fierascu R, Fierascu I, Baroi A, Ortan A, Selected Aspects Related to Medicinal and Aromatic Plants as Alternative Sources of Bioactive Compounds. Int. J. Mol. Sci. (2021); 22(4): 1521.

Kadiri HE, Apiamu A, Aframomum melegueta: a stimulator of liver function enzymes and a down-regulator of cyanide-mediated oxidative injuries in rats, Sci World J. 2022; 17(3): 375-337.

Chaves N, Santiago A, Alías JC, Quantification of the antioxidant activity of plant extracts: Analysis of sensitivity and hierarchization based on the method used, Antioxidant 2020; 9(1):76.

Kadiri HE, Asagba SO, The chronic effects of cyanide on oxidative stress indices in the domestic chicken (Gallus domesticus L.), The J. Basic and Appl, Zoo. 2019; 80:30.

Anigboro AA, Avwioroko OJ, Ohwokevwo OA, Pessu, B. and Tonukari NJ; Phtyochemical profile, antioxidant, α-amylase inhibition, binding interaction and docking studies of Justicia carnea bioactive compounds with α-amylase. Biophys. Chem. 2021; 269:106529.

Guglani A, Pandey HK, Rajeshwar KK, Arya, Bala M, In-Vitro Antioxidant activity, total Phenolic, Flavonoid and Tannin Contents in the Ajuga Bracteosa Wall. Ex Benth, Grown at Middle Hill Climatic Condition of Western Himalayas, Def Life Sci J. 2020; 5(3):198-203.

Anigboro AA, Avwioroko OJ, Ohwokevwo OA. Pessu B, Bioactive components of Ficus exasperata, Moringa oleifera and Jatropha tanjorensis leaf extracts and evaluation of their antioxidant properties. EurAsian J of Biosci. 2019; 13:1763-1769.

Poorna RR, Anu J, Sathiyajith JN, Phytochemical profiling, In Vitro antioxidant activity of Euphorbia hirta extracts and In Silico study targeting human peroxiredoxin 5 Receptor, Curr Trends in Biotech and Pharm. 2022; 16(3): 344 – 356.

Adnan M, Chy NU, Kamal ATM , Azad MOK, Paul A, Uddin SB.. Investigation of the biological activities and characterization of bioactive constituents of Ophiorrhiza rugosa var. prostrata (D. Don) & Mondal Leaves through In-Vivo, In Vitro, and In Silico Approaches. Mol., 2019; 24:1367.

Ekayoda O, Kadiri HE. Ohwokevwo, OA Combine effects of cadmium-and-cyanide-contaminated diet on oxidative stress biomarkers in different tissues of rats. Gal Med J. 2022; 29(4): E202244.

Mordi J, Achuba FI, Ichipi-Ifukor P, Emete G, Mokogwu TH, Nmanedu AC, Aruoren O, Ohwokevwo AO, Protective influence of Costus afer aqueous extract on biochemical indices of rats fed crude oil contaminated diet Iraqi J. Sci. 2021; 62(12): 4639-4648.

Emordi JE, Agbaje EO, Oreagba IA, Iribhogbe IO, Antidiabetic and hypolipidemic activities of hydroethanolic root extract of Uvaria chamae in streptozotocin induced diabetic albino rats, BMC Complem and Altern Med. 2016; 16:468.

Omale J, Ebiloma UG, Idoko GO, Uvaria chamae (Annonaceae) plant extract neutralizes some biological effects of Naja nigricollis snake venom in rats, Brit. J. Pharmacol. and Toxicol. 2013; 4(2): 41-50.

Ejeh SA, Abalaka SE, Usende IL, Alimi YA, Oyelowo FO, Acute toxicity, oxidative stress response and clinicopathological changes in Wistar rats exposed to aqueous extract of Uvaria chamae leaves Sci Afri. (2019); 3(2019)e00068.

Abu T, Rex-Ogbuku EM. Idibiye KA, review: secondary metabolites of Uvaria chamae p. Beauv. (Annonaceae) and their biological activities. Int. J. Agric. Environ. and Food Sci. 2(4):177-185.

Basil NI, Antioxidant Activity of Cnestis ferruginea and Uvaria chamae seed extracts. Brit J Pharm Res, 2017; 16(1), 1-8.

Okwu DE, Iroabuchi F. Phytochemical composition and biological activities of Uvaria chamae and Clerodendoron splendens. E-journal Chem. 2009; 6(2):553-60.

Madubunyi II, Onoja SO, Asuzu IU. In vitro antioxidant and in vivo antidiabetic potential of the methanol extract of Ficus glumosa Del (Moraceae) stem bark in alloxan-induced diabetic mice. Compar clin. pathol. 2012 21:389-94.

Borokini, TI, Omotayo FO,. Comparative phytochemical analysis of selected medicinal plants in Nigeria. Int. J. Adv. Chem. Res. 2012; 1(1): 011-018.

Njoku OV,Chidi O, Phytochemical constituents of some selected medicinal plants, Afri. J. Pure and Appl. Chem. 2009; 3(11): 228-233.

Singleton VL, Rossi JA, Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents, Americ. J. Enolo and

Viticul.1965; 16: 144-158.

Jia Z, Tang M, Wu J, The Determination of Flavonoid Contents of Murlberry and Their Scavenging Effects on Superoxide Radicals, Food Chem.1999; 64:555-559.

Shamsa F, Monsef H, Ghamooshi R, Verdian-rizi M. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J. Pharmaceut. Sci. 2008;32(1):17-20.

Oruka O, Achuba FI. In vitro antioxidant and anti-inflammatory activities of aqueous leaf extract of Alchornea cordifolia. JASEM. 2023 28;27(2):299-304.

Marcocci I, Marguire JJ, Droy-lefaiz MT, Packer L, The nitric oxide scavenging properties of Ginkgo biloba extract, Biochem Biophysi Res Comm. 1994; 201: 748–755.

Oyaizu M, Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucosamine, J Nutr.1986; 44: 307-315.

Prieto P, Pineda M, Aguilar M, Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E, Analyt Biochem. 1999; 269(2): 337-341.

Oyedapo OO, Famurewa AJ, Antiprotease and membrane stabilizing activities of extracts of Fagara zanthoxyloides, Olax subscorpiodes and Tetrapleura tetraptera. Int. J Pharmacog. 2008; 33(1): 65-69.

Eshwarappa RS, Ramachandra YL, Subaramaihha, SR, Subbaiah SG, Austin RS, Dhananjaya BL, Anti- lipoxygenase activity of leaf gall extracts of Terminalia chebula (gaertn.) retz. (Combretaceae). Pharmacogn Res. 2016; 8(1):78–82.

Tseha ST, Mekonnen Y, Desalegn A, Eyado A, Wondafarsh M, Anti-Inflammatory and Phytochemical Analysis of the Crude Leaves Extracts of Boscia Coriacea Graells and Uvaria Leptocladon Oliv. Ethiopian J health Sci. 2022; 32(4): 817–822.

Iwetan BB, Obianime AW, Ewhre LO, Kweki GR, The Antioxidant Modulating Properties of Justicia carnea Extract on Sheep Red Blood Cells Immunized Mice, J. Pharmaceut. Res. Int. 2022; 34(33B): 58-74

Kweki GR, Ichipi-Ifukor PC, Asagba SO, High caffeine-containing energy drink-induced metabolic stress in rats, Sokoto J. Med. Lab. Sci. 2018; 3 (3): 83-93

Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V , Kohli K, Mechanism of action of flavonoids as anti-inflammatory agents: a review, Inflam & Allergy—Drug Targets. 2009; 8(3):229–235.

Mohammed MS, Osman WJA, Garelnabi A E, Osman Z, Osman B, Khalid HS, Mohamed MA,. Secondary metabolites as anti-inflammatory agents, J. Pharmaceut. Sci. and Pharmacol. 2014; 3(4): 275–285

Thawabteh A, Juma S, Bader M, Karaman D, Scrano L, Bufo SA, Karaman R. The biological activity of natural alkaloids against herbivores, cancerous cells and pathogens. Toxins. 2019; 11;11(11):656.

Khan I, Khan A, Halim SA, Saeed A, Mehsud S, Csuk R, Al-Harrasi A, Ibrar A. Exploring biological efficacy of coumarin clubbed thiazolo [3, 2–b][1, 2, 4] triazoles as efficient inhibitors of urease: A biochemical and in silico approach. Int. J. biol macromol. 2020; 1; (142):345-54.

Mafa PJ, Malefane ME, Idris AO, Liu D, Gui J, Mamba BB, Kuvarega AT. Multi-elemental doped g-C3N4 with enhanced visible light photocatalytic activity: insight into naproxen degradation, kinetics, effect of electrolytes, and mechanism. Separation and Purification Technol. 2022; 1;(282):120089.



How to Cite

Kweki, G. R., Orhu, A., Uzuegbu, U., Iwhiwhu, O. S., & Ohwokevwo, O. A. (2024). <i>In-vitro</i> Anti-inflammatory and Antioxidant Potentials of Methanol Extract of&nbsp;<i>Uvaria chamae</i>&nbsp;(Bush Banana) Leaves: http://www.doi.org/10.26538/tjpps/v3i1.6. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 3(1), 153–157. Retrieved from https://www.tjpps.org/index.php/home/article/view/40