Inhibitory Effects of n-Hexane Extracts of <i>Sida acuta</i> and <i>Aspilia africana</i> Leaves on Platelet Aggregation
http://www.doi.org/10.26538/tjpps/v3i1.3
Keywords:
Inhibitory, Aspirin, aggregation, platelet, Aspilia africana, Sida acutaAbstract
Platelet hyperactivity significantly contributes to the pathogenesis of arterial thrombosis and atherosclerosis. The primary objective of this investigation was to assess the antiplatelet activity of the n-Hexane leaf extract of Sida acuta and Aspilia africana leaves using in vitro experiment. Standard analytical procedures were employed using UV-Vis spectrophotometric method. Three concentrations (0.5mg/ml, 2mg/ml and 4mg/ml) of Sida acuta and Aspilia africana leaf extracts were utilised. Aspirin (2mg/ml) and distilled water serving as positive and negative controls, respectively. Platelet aggregation was induced using 10% w/v Calcium chloride. The findings demonstrated that the n-Hexane leaf extract of Sida acuta exhibited a statistically significant antiplatelet activity of 55.00 ± 0.46, 55.45 ± 0.91 and 55.30 ± 0.95 while Aspilia africana extract displayed antiplatelet activities of 47.64±0.65, 51.41±1.13, and 51.74±0.29 at concentrations of 0.5mg/ml, 2.0mg/ml, and 4.0mg/ml, respectively, surpassing the antiplatelet activity of Aspirin, which was measured at 39.83%. The findings suggest that the extracts of the leaves of Sida acuta and Aspilia africana possess significant antiplatelet activity.
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Smith SC, Collins A, Ferrari R, Holmes DR, Logstrup S, McGhie DV, Ralston J, Sacco RL, Stam H, Taubert K, Wood DA, Zoghbi WA. Our time: a call to save preventable death from cardiovascular disease (heart disease and stroke). J. Am Coll Cardiol 2012; 60(22):2343-2348
Dela Torre S. DOH: Heart attack, stroke and cancer remain top causes of death among Filipinos. Throm Haemost. 2014; 89(4):610-621
Wagner DD, Burger PC. Platelets in inflammation and thrombosis. Arterioscler Thromb Vasc Biol. 2003; 23(12):2131-2137
Furie B, Furie BC. Mechanisms of thrombus formation. New Eng. J. of Med. 2008; 359:938-949
Chen JJ, Chang YL, Teng C M, Cheng IS. Anti-platelet aggregation alkaloids and ligands from Hernandia nymphaeifolia. Planta Med. 2000; 66:251-256
Chua TK, Koh HL. Medicinal Plants as potential sources of lead compounds with anti-platelet and anti-coagulation activities. Mini-Re. Med. Chem. 2006; 6:611-624
Watkins NA, Gusnanto A, De Bono B, De S, Miranda-Saavedra D, Hardie DL, Haem A. Characterizing gene expression in differentiated human blood cells. Blood 2009; 113:1-9
Palankar R, Kohler TP, Krauel K, Wesche J, Hammerschmidt S, Greinacher A. Platelets kill bacteria by bridging innate and adaptive immunity via platelet factor 4 and FcᵧRIIA.Thromb. Haemost. 2018; 16(6):1187-1197
Agiang EA and Oko OO. Antimicrobial activities of crude extracts of Aspilia africana leaves. Anim. Sc. Ass. of Nig. (2010); 15:123-125
Catella-Lawson F. Cyclooxygenase inhibitors and the antiplatelet effects of Aspirin. N Engl J Med 2001; 345:1809-1817
Kayode J. Conservation of indigenous medicinal botanicals in Ekiti State, Nigeria. J. Zhejiang Univ. Sci. 2006; 7(9):713-718
Holm LG, Plucknett D L, Pancho JV, Herberger JP. The world’s worst weeds: distribution and biology. Univ. of Hawaii press, Honolulu; 1977; 89(4):610-621
All Point Bulletin. (APB). Sida. Agriculture Protection Board of Western Australia Advisory Leaflet, 1983; 94:2
Flanagan GJ, Hills LA, Wilson CG. The successful biological control of spiny head Sida acuta, by Calligrapha pantherina (Col: Chrysomelidae) in Australia’s Northern Territory. In: Proceedings of the X Intl Symposium on Biol Contrl of weeds, Bozeman, Montana. Bozeman; 2000; 35-41
Karou D, Dicko MH, Simpore J, Traore AS. Anti-malarial activity of Sida acuta BURMF L. (Malvaceae) and Ptero-carpus erinaceus POIR (Fabaceae). J. Ethnopharmacol. 2005; 89:291-294
Otero R, Nunez V, Barona J, Fonnegra R, Osorio RG, Garcia, ME, Diaz A. Snakebites and ethnobotany in the northwest region of Colombia. Part III: neutralization of haemorrhagic effect of Bothrops atrox venom. Ethnopharmacol. 2000; 73:233-241
Saganuwan S, Gulumbe ML. Evaluation of Sida acuta subspecie acuta leaf/flower combination for antimicrobial activity and phytochemical constituents. Afri. J. of Clinic and Experi. Microbiol. 2006; 7(2):83-88
Caceres A, Giron LM, Martinez AM. Diuretic activity of plants used for the treatment of urinary ailments in Guatemala. J. Ethnopharmacol. 1987; 19:233-245
Ignacimuthu S, Ayyanar M, Sankara-Sivaramann K. Ethnobotanical investigations among tribes in Madurai District of Tamil Nadu (India). J. Ethnobiol. Ethnomed.2006; 2:25
Dalziel JM. The useful plants of West Africa. Crown Agents, London. 1973; 18- 25
Akujobi CO, Ogbulie JN, Okorondu T. Antibacterial and nutrient potentials of Gongronema latifolium and Piper guineensis used in herbal remedies and as species. Niger. J. Microbiol., 2004; 18(1-2):241-246
Iwu MM. Handbook of Medicinal Plants. CRP Press, Florida. 31-1993
Adjanohoum JE, Aboubakar N, Dramane K, Ebot ME, Ekpere JA, Enow-Orock EG, Focho D, Gbile ZO, Kamanyi A, Kamsu kom J, Keita A, Mbekum T, Mbi CN, Mbielle AL, Mbome IL, Mubiri N K, Nancy WL, Nkongmeneck B, Satabie B, Sofowa A, Tamze V, Wirmum CK. Trad.medic. and pharmacopeia-contri. to ethnobotanical and floristic stud. in Cameroon CNPMS,Porto-Novo, Benin .1996; 20-25
Oliver BE. Medicinal plants in Nigeria. Nigerian College of Arts, Science and Technology, Lagos Nigeria. 1960;12-26
Sofowora EA. Research on medicinal plants and traditional medicine in Africa. J Altern Complement Med. 1996; 2(3):365-372
Page JE, Baiza F, Nishida T, Towers GH. Biologically active diterpenes from Aspilia mossambicienesis- a chimpanzee medicinal plant. Phytochem. 1992; 31(10):3437-3439
Abi TA, Onuoha EN. The chemical constituents of the leaf of Aspilia africana as a scientific backing to its tradomedical potentials. J Agric Sci. 2011; 6(1):28-30
Okoli CO, Akah PA, Nwafor SV, Asiniobi A J, Ibegbunam, IN, Erojikwe O. Anti-inflammatory activity of hexane leaf extract of Aspilia africana. J. Ethnopharmacol., 2007; 109(2):219-225
Macfoy CA, Cline EI. In vitro antibacterial activities of three plants use in traditional medicine in Sierra Leone. J. Ethnopharmacol. 1990; 28:323-327
Oyedapo OO, Akindele VR, Okunfolami OK. Effects of extracts of Olax subscorpioides and Aspilia africana leaf on bovine red blood cells. Phytother. Res. 1998; 19:633-642
Obute GC, Adubor GO. Chemicals detected in plants used for folk medicine in Southeastern Nigeria. http://www.siu.edu/hebl/leaflets/obute.html.2005; 2-6
Chen D, Yi X, Yang H, Zhou H, Yu Y, Tian Y. Genetic diversity evaluation of winged bean ((Psophocarpus tetragonolobus (L.) DC.)) using inter-simple sequence repeat (ISSR). Genet. Resour. Crop Evol. 2015; 62(6):823-828
Whalen K, Finkel R, Panavelil T. Lippincott illustrated reviews. J. Pharmacol 2015; 120(3):291-301
Wong Gary, Wenjun Liu, Yingxia Liu, Boping Zhou, Yuhai Bi, George F. Gao. MERS, SARS, and Ebola: The Role of Super-Spreaders in Infectious Disease Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China 3Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People’s Hospital, Shenzhen 518112, China 4Office of Director-General, Chinese Centr for Dis. Contrl and Preventn, Beijing 102206, China2015; 34-43.
Whalen K, Finkel R, Panavelil T. Lippincott illustrated reviews. J. Pharmacol 2015; 120(3):291-301
Gerard LL, Juleos AA, Elijah NC, Alterado FJ. In vitro platelet aggregation inhibition activity of Psophocarpus tetragonobolus(L.)DePodExtract.Int. J. Pharmacogn. Phytochem.2017; 9(1):70-75
Oko OO, Agiang EA. Phytochemical activities of Aspilia africana leaves using different extractants. Indian J Anim Sci. 2011; 81(8): 814-818.
Adeniyi BA, Odufowora RO. In-vitro anti-microbial properties of Aspilia Africana (compositae). Agric. J. Biomed. Res. 2000; 3:167-170
Tzeng SH, Ko W, Ko CF, Teng CM. Inhibition of platelet aggregation by some flavonoids. Thrombo. Res. 2000; 64(1):91-100
Keevil JG, Osman HE, Reed JD, Folts JD. Grape juice, but not orange juice or grapefruit juice, inhibits human platelet aggregation. J. Nutr. 2000; 130(1): 53-56
Yun-Xiang Z, Ting-TingY, Liu X, Wei-Fen Z, Jia-Fu W, Ya-Ping, W. Inhibitory effect of propolis on platelet aggregation In vitro. J. Healthc Eng. 2017; 1-6
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