Detannification of <i>Ananas comosus</i> Rinds Juice with Tnnase Immobilized on the Beads of <i>Chrysophyllum albidum</i> Seeds
http://www.doi.org/10.26538/tjpps/v3i1.4
Keywords:
Rhodotorulla mucilaginosa, Tannin, Ananas comosus, tannase, Chrysophyllum albidumAbstract
Billion tons of agro wastes that constitute hazard to the environment are generated annually all over the world. How to curb this menace remains a major challenge in the field of biotechnology. In this study, a novel immobilization material for microbial tannase was developed from Chrysophyllum albidum seeds. Optimization studies for effective performance of the novel immobilization material were conducted. The characterized immobilized tannase was explored for the detannification of tannin present in Ananas comosus rinds juice. Activities of free and immobilized tannase from R. mucilaginosa CBS 316 were 724.49 and 497.77 U/g/min respectively. Optimal temperature and pH for tannase activity were 35ºC and 7 respectively. Optimum tannase load, bead size and bead number for immobilized tannase were 35%, 8mm and 20 respectively. Immobilized tannase was reusable for up to 3 times. MgSO4, KCl, and ZnSO4 enhanced tannase activity while PbCl2, MgCl, BaCl2 and EDTA inhibited the activity of tannase. Tannin content of the three varieties of Ananas comosus rinds juice from Bene, Cotonou and Local source were drastically reduced from 17.8, 16.3 and 5.9 mg/100g to 3.9, 2.9 and 1.8 mg/100g respectively when treated with immobilized tannase. This study established the detannification of Ananas comosus rinds juice with microbial tannase immobilized on the novel beads of Chrysophyllum albidum seeds.
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